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  • Single Molecule Imaging

    Prof. Leake founded and leads the Biological Physical Sciences Institute at the University of York, which brings together scientists researching the biomolecular interactions, biological modelling imaging and quantitation of complex data.

  • Single Molecule Imaging

    Prof. Peterman and team research protein structural and functional dynamics using combinations of optical tweezers and single molecule fluorescent microscopy.

  • Imaging Live Cell Exocytosis

    Mr Bateman is a Link Scientist working with Dr. Lin Wang in the Central Laser Facility (CLF), which contains everything from lasers the size of a room to compact benchtop lasers. Mr Bateman uses lasers with microscopes to do fluorescence imaging for the academic community, who bid for time at the CLF for their research.

  • Light Sheet and Novel Spatial Frequency Imaging

    Prof. Girkin and his multidisciplinary team are interested in applying advanced photonics and optical technology to challenges within the life sciences, in this case imaging live zebrafish. By using selective plane illumination microscopy (SPIM), the beating hearts (1) and developing eyes (2) of live zebrafish can be imaged and analyzed.

  • Light Sheet Microscopy at University of Toronto

    Currently the Associate Vice President, International Partnerships at the University of Toronto, Dr. Christopher Yip is the former Director of the Institute of Biomaterials and Biomedical Engineering, and a faculty member in IBBME, Chemical Engineering, and Biochemistry, where he and his team create a variety of experiment-specific microscopy tools.

  • Light Sheet Microscopy, Image Resource Facility

    Greg Perry, from the Image Resource Facility at the University of London, works closely alongside academics such as Dr. Osborn and Dr. Valderrama, to improve imaging across a variety of research applications including long-term live imaging of zebrafish development and 3D organization of prostate cancer cell structures.

  • Live Cell Fluorescence Microscopy

    The lab of Prof. Osman’s at the Ludwig-Maximilian University in München, Germany is interested in understanding the power plants of cells – mitochondria – and the way their functionality and network activity is maintained during cell division.

  • Photon Conversion and Anti-Reflection Coatings: Improving UV Sensitivity Above 200 nm

    The majority of camera sensors are silicon-based, such as CCD sensors. Although silicon-based sensors have high quantum efficiency (QE) in the visible wavelength range, they are much less sensitive to wavelengths in the ultraviolet (UV) range (100-400 nm).

  • EMCCDs: The Basics

    Electron-multiplying CCDs (EMCCDs) are a variant of silicon-based CCDs that use electron multiplication to elevate electron signal greatly above the read noise floor to maximize sensitivity for low-light imaging. Photons are collected on EMCCD sensors in a similar way to CCD sensors, however, the addition of an EM-gain register allows photoelectrons to be amplified before being read out.

  • Pixel Size and Camera Resolution

    A pixel is the part of a sensor which collects photons so they can be converted into photoelectrons. Multiple pixels cover the surface of the sensor so that both the number of photons detected, and the location of these photons can be determined.

  • Scientific Camera Noise Sources

    Variation in the signal resulting in uncertainty in the image data is referred to as noise. It can be produced by the sensor, surrounding electronics, temperature of the system and via natural fluctuation.

  • Signal to Noise Ratio (SNR)

    Signal to noise ratio (SNR) is defined as the relationship between the signal and the noise generated within a pixel. If the sample signal is weak in comparison to the noise associated, it can be difficult to detect.