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Single Molecule TIRF
The Cees Dekker lab at TU Delft works with single-molecule imaging techniques in order to explore life at the nanoscale. Roman Barth is a PhD candidate in the Cees Dekker Lab, running experiments using the two TIRF single-molecule imaging systems in the lab.
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Imaging Live Cell Exocytosis at Saarland University
Dr. Ute Becherer studies the regulation of exocytosis and endocytosis using several different model systems, including the release of vesicles containing adrenaline from chromaffin cells (neuroendocrine cells), synaptic transmission in neurons, and the mechanisms of immune cytotoxic T cells. Proteins involved in regulating exocytosis are also present in T cells, and learning more about the processes behind exocytosis is relevant for entire organisms. Dr. Becherer is studying this exocytosis from the molecular scale to entire in vivo animals.
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Imaging Neurotransmitter Release
The Volynski group are primarily interested in understanding cellular regulation of synaptic release of neurotransmitters which forms the basis of communication among neurons in the brain.The Volynski group uses fluorescent probes, such as vesicular release sensor synaptophysin-pHluorin (sypHy).
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Single-Molecule Imaging
The Krishnan Lab, headed by Prof. Madhavi Krishnan, is involved in the study of soft condensed matter at the nanometre scale, at the University of Oxford. This involves applied physics and photonics, physical chemistry of charged interfaces, and single-molecule imaging.
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Super Resolution
The Laboratory of Experimental Biophysics (LEB) at EPFL, headed by Prof. Suliana Manley, develops and uses fluorescence imaging techniques combined with live-cell imaging and single-molecule tracking to determine how the dynamics of protein assembly are coordinated.
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Microfluidic Live Yeast Imaging
The Rine lab at the University of California, Berkeley is working towards understanding mechanisms underlying establishment, maintenance, and epigenetic inheritance of gene silencing in yeast. The lab has developed a genetic strategy to capture transient losses of gene silencing of heterochromatin in S. cerevisiae, and translating these dynamic processes as a permanent modification of fluorescence expression.
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Novel Hyperspectral Imaging
The interdisciplinary laboratory led by Prof. Silas Leavesley and Dr. Thomas Rich is working to develop novel hyperspectral imaging systems for microscopy and endoscopy. Using rapidly controllable light sources with precise spectral selection they aspire to be able to increase the number of individual sensors and probes detected concurrently.
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High Content Multiplex Fluorescence
Dr Sonia Leonardelli of the Hölzel Lab performs fluorescent imaging of a range of different tumour tissue samples, one of the main projects being to study cell-cell interactions in adenocarcinoma. The fluorescence imaging in this lab using the CODEX® system from Akoya Biosciences® with a Zeiss microscope in order to take multiple fluorescence images with multiple different fluorophores, which can then be collated and output with all the fluorophores in the same image, up to 60 different types.
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Light Sheet Microscopy at Morgridge Institute for Research
Dr. Jan Huisken, Director of Medical Engineering at the Morgridge Institute for Research has been instrumental in the invention and development of Light Sheet Microscopy. One of his current research interests is in the development of novel methods to image cleared tissues.
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Live Cell Imaging at Heidelberg University Medical School
The Frischknecht lab aims to understand transmission of the malaria parasite between host and mosquito. The lab focuses on the two motile stages of the life cycle – the ookinete and the sporozoite using the rodent model parasite Plasmodium berghei.
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Imaging With A Quantum Light Source
Dr. Gräfe and Ms Gilaberte-Basset’s research and development centres around quantum imaging light sources. The group is currently building a new light source that will make use of quantum imaging to permit excitation of samples in the UV range, whilst detecting in the visible range, through the manipulation of correlated photons.
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Single-Molecule Biophysics
The Freedman Lab studies single molecule biophysics using a variety of electrical and optical signal measurements. Most notably, the lab studies complex biological systems where molecular populations are heterogeneous and difficult to study using ensemble averaging.