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  • Analysis Programs For High Content Imaging

    High-content imaging (HCI) involves a powerful imaging system paired with smart analysis software, in order to parse hundreds of thousands of dense images into quantifiable data. As HCI involves maximizing the data output, HCI experiments can involve imaging millions of cells with multi-parameter analysis, resulting in the need for efficient, often automated, smart specialized analysis software.

  • Resolution and Numerical Aperture

    An often-asked question in imaging is whether two objects are in the same or separate places. Resolution, the ability to tell two nearby features apart, is a key parameter of microscope optics that becomes more challenging at smaller length scales.

  • Introduction To High Content Imaging

    High content imaging (HCI) is an area of imaging where the aim is to maximize data capture. Any kind of imaging can be high-content if the objective is to obtain as much data as feasibly possible, regardless of imaging system, sample, magnification, fluorophores, and camera used. This makes specifics in HCI difficult to define, but in general, HCI involves performing normal imaging thousands or millions of times in order to maximize data capture effectively.

  • Total Internal Reflection Fluorescence (TIRF) Microscopy

    Fluorescence microscopy is a fundamental set of techniques in the life sciences for visualizing structures in living systems. Typically, a fluorescent molecule, either synthetic or biological, is associated with a structure of interest in a biological sample.

  • Super-Resolution Radial Fluctuations (SRRF)

    Some super-resolution data cannot be visualized directly, only after images are reconstructed and processed can they be displayed, such as in PALM and STORM. This processing requires specialized algorithms and analysis software, which must be capable of dealing with the large datasets of thousands of frames from 3D, fast-moving dynamic samples with hundreds of data points of varying densities.

  • Intravital NIR Imaging

    The group of Prof. Gousopoulos at the University Hospital Zurich is focused on researching lymphedema, a condition where lymphatic system dysfunction results in swelling in parts of the body. The group has established a mouse model in order to investigate this disease.

  • Microfluidics and Live Cell Imaging

    The deMello Group at ETH Zürich is engaged in a broad range of activities in the general area of microfluidics and nanoscale science. Primary specializations include the development of microfluidic devices for high-throughput biological and chemical analysis, ultra-sensitive optical detection techniques

  • Single Molecule Micromirror TIRF

    The Duderstadt Group are interested in understanding the organization and dynamics of macromolecular complexes, such as the replisome which is responsible for DNA replication.Successful DNA replication is critical for cell survival, and errors within this process have been implicated in many disease pathologies.

  • Nanophotonics

    Dr. Mivelle’s research lies within the field of nanophotonics and centers around investigations into the interactions between light and matter on the nanoscale to increase understanding of optical properties.

  • Kinetix22 Pre-Arrival Guide

    Thank you for your interest in the Kinetix22 sCMOS, the next generation in scientific CMOS cameras. This is a guide to help you prepare for the arrival of the Kinetix22, ensuring that the hardware and software of both your PC and microscope/imaging system are capable of making the most of the power of the Kinetix22 and delivering your desired data.

  • Single Molecule Spectroscopy at Heidelberg University

    Professor Herten leads the Single Molecule Spectroscopy group at Heidelberg University aiming at quantitative analytical approaches in biology and chemistry based on single-molecule data acquired with advanced fluorescence microscopy.The groups research interests range from the development of fluorescent probes for live-cell microscopy to the investigation of chemical reactions on a single-molecule level.

  • Single Molecule Tracking PALM

    The Carr Lab at the Genome Damage and Stability Centre, University of Sussex, investigates DNA metabolism processes such as DNA replication and repair. They are interested in the challenges cells face during DNA replication and the cellular processes that help the cell overcome replication fork stalling or collapse.