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Widefield Fluorescence Microscopy
Professor Steven Pittler, Director of the Vision Science Research Center at the University of Alabama at Birmingham, uses fluorescence imaging to study the diseases related to vision. Dr. Pittler explains, “Our Molecular and Cellular Analysis Core focuses on the investigation of a range of ocular diseases in the anterior and posterior segments of the eye.
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Single Molecule Imaging at University of Lund
The Linke Group at the University of Lund, Sweden, creates artificial molecular motors and nanowires to better understand the role of biological motors in cellular processes such as cargo transportation, muscle contraction and cell division.
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TIRF Microscopy, Dr. Shiaulou Yuan
The Martina Brueckner lab at Yale University School of Medicine studies the genes of children with congenital heart disease. “We take a human genetics approach combined with animal models, such as mouse and zebrafish, and in vivo imaging.
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Single Molecule Imaging
Prof. Leake founded and leads the Biological Physical Sciences Institute at the University of York, which brings together scientists researching the biomolecular interactions, biological modelling imaging and quantitation of complex data.
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Single Molecule Imaging
Prof. Peterman and team research protein structural and functional dynamics using combinations of optical tweezers and single molecule fluorescent microscopy.
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Imaging Live Cell Exocytosis
Mr Bateman is a Link Scientist working with Dr. Lin Wang in the Central Laser Facility (CLF), which contains everything from lasers the size of a room to compact benchtop lasers. Mr Bateman uses lasers with microscopes to do fluorescence imaging for the academic community, who bid for time at the CLF for their research.
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Light Sheet and Novel Spatial Frequency Imaging
Prof. Girkin and his multidisciplinary team are interested in applying advanced photonics and optical technology to challenges within the life sciences, in this case imaging live zebrafish. By using selective plane illumination microscopy (SPIM), the beating hearts (1) and developing eyes (2) of live zebrafish can be imaged and analyzed.
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Light Sheet Microscopy at University of Toronto
Currently the Associate Vice President, International Partnerships at the University of Toronto, Dr. Christopher Yip is the former Director of the Institute of Biomaterials and Biomedical Engineering, and a faculty member in IBBME, Chemical Engineering, and Biochemistry, where he and his team create a variety of experiment-specific microscopy tools.
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Light Sheet Microscopy, Image Resource Facility
Greg Perry, from the Image Resource Facility at the University of London, works closely alongside academics such as Dr. Osborn and Dr. Valderrama, to improve imaging across a variety of research applications including long-term live imaging of zebrafish development and 3D organization of prostate cancer cell structures.
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Live Cell Fluorescence Microscopy
The lab of Prof. Osman’s at the Ludwig-Maximilian University in München, Germany is interested in understanding the power plants of cells – mitochondria – and the way their functionality and network activity is maintained during cell division.
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Photon Conversion and Anti-Reflection Coatings: Improving UV Sensitivity Above 200 nm
The majority of camera sensors are silicon-based, such as CCD sensors. Although silicon-based sensors have high quantum efficiency (QE) in the visible wavelength range, they are much less sensitive to wavelengths in the ultraviolet (UV) range (100-400 nm).
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EMCCDs: The Basics
Electron-multiplying CCDs (EMCCDs) are a variant of silicon-based CCDs that use electron multiplication to elevate electron signal greatly above the read noise floor to maximize sensitivity for low-light imaging. Photons are collected on EMCCD sensors in a similar way to CCD sensors, however, the addition of an EM-gain register allows photoelectrons to be amplified before being read out.