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Super Resolution Bacterial Imaging
Dr. Holden’s research lies between biophysics and microbiology, using super-resolution microscopy to study basic principles of bacterial spatial organization. In particular, the Holden Lab focuses on how the Gram-positive model bacterium Bacillus subtilis divides, and how the bacterial cytoskeleton guides the construction of a mid-cell cross-wall or septum.
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Pattern Noise: DSNU and PRNU
The sensitivity of a scientific camera is vital, with insufficient sensitivity it may not even be possible to acquire clear images of your sample. At Teledyne Photometrics sensitivity is paramount and is our approach to highly sensitive cameras is twofold: maximize signal collection, and minimize noise levels.
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Super-Resolution Microscopy at The Laboratory of Experimental Biophysics EPFL
Dr. Kyle Douglass, a research scientist at the EPFL, has spent the past several years developing high-throughput and automation methods for super-resolution fluorescence microscopy. The Laboratory of Experimental Biophysics, which is led by Prof. Suliana Manley, uses these techniques to study the structural biology of multi-protein complexes such as chromatin foci, the bacterial division machinery, and the centrosome.
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Triggering
As discussed in our previous article on rolling vs global shutters, our camera range uses electronic shutters, meaning the sensor is continuously exposed with no mechanical shutter or aperture to block light to the sensor. These cameras continuously read out the sensor when in operation, and can produce an image upon request by software, which is the image you see on your computer.
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Spectral Optical Coherence Tomography
The Verhoef Lab designs novel laser light sources to enhance existing and support novel imaging methods. Their systems might result in signals ranging from the detection of a few photons for super-resolution imaging, through to differentiating small differences amongst many tens of thousands of photons.
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Single Molecule Dynamics
Dr. Deshpande’s research focuses on the use of synthetic biology approaches to study real-time dynamics of biomolecules.
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Calcium Imaging at Freie University, Berlin
Emil Kind is a PhD student in the lab of Prof. Wernet, which focuses on neural circuitry, especially circuits involved in navigation and orientation behaviour. Their studies span from looking at neuroanatomy on a cellular level to behaviour on the organism level using the fruit fly Drosophila as a model system.
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Why Photometrics?
Teledyne Photometrics provides scientific CMOS, EMCCD, and CCD camera solutions that turn your experiments into results.Our products are widely used to support demanding, quantitative research, from live animal imaging to quantum physics.
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Camera Resolution
In imaging, the resolution is defined as the shortest distance between two points on a specimen that can still be distinguished. This means that if a camera has a resolution of 1 µm, it can resolve objects in the sample that are more than 1 µm apart. But if objects are closer than 1 µm the imaging system will be unable to tell them apart, and they will appear as one object.
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Single Molecule TIRF
Dr. Pradhan’s research is concerned with understanding how DNA supercoils with a family of proteins called structural maintenance of chromosome (SMC) proteins.
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smFRET, STORM and TIRF
The Hohlbein Lab uses techniques such as single molecule imaging, FRET and super-resolution PALM/STORM to investigate a variety of topics.
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Camera Sensitivity
Camera sensitivity is one of the most important aspects of camera performance – with inadequate sensitivity, your imaging experiment may simply be impossible. However, sensitivity is a broad topic dependant on a large number of factors, and cannot be represented by one value alone – while one camera may outperform another at one light level or the exposure time, the situation may be reversed at higher light or longer exposures.