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  • Low Noise Imaging in the GS3-U3-15S5 Camera

    This application note describes the low noise imaging in the GS3-U3-15S5 camera, including: Description and use of optimized mode. Comparison of imaging metrics, including saturation capacity, temporal dark noise, and temperature, between standard and optimized imaging modes. Comparison of images between standard and optimized modes.

  • Optimized Modes for Low Light Imaging in the Grasshopper2 FireWire Camera

    This application note describes the optimized modes for low light imaging available in Grasshopper2 FireWire cameras, including: 1. Description and use of optimized modes. 2. Comparison of imaging metrics, including full well depth, read noise, dark noise, dark current and temperature, between standard and optimized imaging modes. 3. Comparison of images between standard and optimized modes.

  • Overview of the Ladybug Image Stitching Process

    The purpose of this Technical Application Note is to: Explain how the Ladybug API creates a single panoramic image from six separate raw images that are output from a Ladybug camera. Explain why stitching is an imperfect process and how to work with stitching errors.

  • Saving Custom Settings on FLIR Machine Vision Cameras

    This application note describes how to save custom image settings onto a FLIR machine vision camera. Using FLIR machine vision software (whether through a GUI or through working directly with our API), it’s possible to change a variety of settings, such as frame rate, region of interest, pixel format, or gain. The complete list of settings that are stored are found in your camera's Technical Reference manual, available from the downloads page. By default, once a camera has been power-cycled (disconnected from its power source and reconnected), the camera starts up with its factory default settings. Using the FlyCapture®2 SDK, or the Spinnaker® SDK, it’s possible to save custom settings to the camera so that even after a power-cycle occurs, the camera starts up with the settings that were saved. Each camera is capable of saving up to two custom profiles.

  • Saving Images at High Bandwidth

    This Technical Application Note provides an analysis of the challenge of saving images at high bandwidth and offers methods to solve the issues. The FlyCapture2 SDK includes a GUI application (FlyCap2) for capturing and saving images as well as an API for writing applications. Using one of FLIR’s fastest Grasshopper3 USB 3.1 cameras, we demonstrate how to stream and save images to disk at a speed of 373 MB/s.

  • Spinning Disk Confocal In Vivo Imaging

    The Johns Hopkins University School of Medicine Microscope Facility has an expansive selection of research microscopes, including multiple laser scanning confocal microscopes, a multiphoton microscope, an atomic force microscope, a TIRF microscope and multiple spinning disk confocal microscopes.

  • Super Resolution Microscopy (SptPALM/STORM)

    Research at the Nanoorganization Lab, Centre for Neuroscience, Indian Institute of Science in Bangalore India borders on the interface of single molecule spectroscopy and molecular and cellular neuroscience. Here, team members develop and adapt state of the art paradigms in ultra-high and super resolution microscopy to image molecules at the synapses of living neurons.

  • Single Molecule Spectroscopy at Sussex University

    Research at Sussex University focuses on the development and application of ultrasensitive optical techniques for the detection and manipulation of single molecules. Representing the ultimate level of sensitivity in the analysis and control of matter, single molecule techniques have many advantages over conventional ensemble methods, namely the measurement of static and dynamic heterogeneity in molecular systems.

  • Single Molecule and TIRF

    The School of Chemistry has a long history of excellence in both research and teaching. It maintains a superb research environment with world leading research groups and facilities.

  • Electrophysiology and Calcium Imaging

    The Department of Human Physiology was established in 1974 as the first department in the School of Medicine at Flinders University. The department has a research focus in neurosciencein three major areas; sensory and autonomic neurobiology, roles of neurotrophic factors and neurodegenerative diseases.

  • Live Cell Imaging, Membrane Dynamics

    The ability to perceive external stimuli and respond accordingly is a fundamental characteristic of biological systems. The stimuli are then converted to signals that can be read by cells via the process of signal transduction, of which there are many different kinds.

  • Optimizing Detection in Whole Animal In vivo Imaging

    Animal studies contribute significantly to our understanding of human disease, and function as an established and essential step in the development of treatments and other therapeutic agents. These studies are conducted in the preclinical phase, preceding drug screening in human clinical trials.