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What Is Live Cell Imaging?
An enormous amount of life science research involves imaging cells/tissues of all kinds. There are two main paradigms when imaging cells and tissues: fixed or live.
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Ultra-High-Speed, Time-Resolved SRS Spectroscopy in Combustion
In combustion, until recently only two temporal optical gating schemes were available to increase signal-to-noise ratio (SNR) for time-resolved spontaneous Raman scattering (SRS) spectroscopy. Problematic optical background noise could be rejected either by electronic gating with an image intensifier or by using a mechanical shutter. Unfortunately, each of these traditional approaches has its shortcomings.
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Confocal Raman Microscopy Study of Biological Tissues
Over the past decade, significant progress has been achieved in the development of high-precision spectrographs, scientific camera technologies, and novel data analysis algorithms.
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Paleoluminescence
Paleontologists such as Dr. Pierre Gueriau rely on the accurate observation and interpretation of fossil anatomy in order to elucidate the origin and evolutionary history of life.
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Calcium Imaging at Heidelberg University
Dr. Freichel’s group are primarily interested in how ion channels regulate the influx of calcium ions (Ca2+) as messengers for cellular and systemic functions.
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Calcium Imaging at Ecole des Mines de Saint-Etienne
Professor Rod O’Connor and Dr. David Moreau work together to develop flexible, conductive polymer electrode devices to record electrical activity and optical measures of physiology from neurons to study the bioelectrical basis of diseases like epilepsy, Alzheimer’s disease and cancer.
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Spinning Disk Expansion Microscopy
The laboratory of Prof. Ewers moves in a number of different research directions, one of these deals with the septin cytoskeleton. Septins are a family of essential, conserved GTP-binding proteins that form heteromeric, non-polar complexes that further assemble into filamentous structures.
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PLIF Combustion Imaging
The group of Prof. Dirk Geyer, including Ph.D. students Martin Richter and Adrian Breicher, work towards the decarbonization of energy conversion. They told us about their research, “Our main research field is the combustion of promising new fuels for the future such as hydrogen and ammonia, which, unlike methane, contain no carbon in their molecular structure and therefore produce no CO2 emissions in the combustion process.
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3D Axially Swept Light-Sheet Microscopy
The lab of Prof. Reto Fiolka develops new, transformative technologies to image across scales: from sub-cellular imaging to imaging of whole organs. In the Fiolka Lab, Dr. Stephan Daetwyler is a postdoctoral researcher who builds, programs and applies advanced light-sheet microscopy systems to image dynamic processes in live biological organisms.
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Fluorescence Correlation Spectroscopy
The lab of Prof. Enrico Gratton at the University of California, Irvine, is interested in the dynamics of the cell interior. The group investigates this using microscopy combined with mathematical approaches such as fluorescence correlation spectroscopy.
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Simultaneous Multichannel SMLM
Both Dr Ian Dobbie and Dr Jingyu Wang work with advanced optics and imaging systems at the University of Oxford. One of their projects aims to achieve the maximum possible 3D imaging resolution in multicolour single-molecule localisation microscopy (SMLM).
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iSCAT and TIRF
Dr. Francesco Reina is a postdoc in the lab of Prof. Christian Eggeling, taking part in quantitative imaging research. The lab of Prof. Eggeling is known for the application of fluorescence correlation spectroscopy (FCS) and STED-FCS to life sciences research, and Dr. Reina is building an imaging system that combines imaging techniques involving single-molecule tracking, namely interferometric scattering (iSCAT) and total internal reflection fluorescence (TIRF) microscopy.