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  • Single Molecule FRET

    Prof. Ehud Isacoff’s lab at the University of California, Berkeley, USA is interested in the mechanistic properties of metabotropic and ionotropic receptor subunits and the role the resultant protein complexes play in signal transduction. His group uses a combination of microscopy approaches including single molecule Föester resonance energy transfer (smFRET) in live cells, and in vivo imaging in a variety of species.

  • Single Molecule Imaging

    TheLeeLab, at the University of Cambridge, focuses its research on developing biophysical tools to answer fundamental biological questions, primarily using single-molecule fluorescence imaging techniques.

  • mesoSPIM

    The mesoSPIM initiative (mesospim.org) is an open science project aimed at making light-sheet microscopes for imaging large cleared tissue samples more accessible to the imaging community. The acronym mesoSPIM stands for “mesoscale selective plane illumination microscopy” and the project was started in 2015 by Dr. Fabian Voigt and Prof. Fritjof Helmchen at the Brain Research Institute of the University of Zürich.

  • Bit Depth, Full Well, and Dynamic Range

    Cameras are a vital technology in scientific imaging, working as an interface between light and a digital image. There are a number of steps involved to convert photons of signal from your sample to the image you see on your computer monitor, each step has variables and factors that can change the ways in which images are generated.

  • The Retiga ELECTRO For Electrophysiology

    Electrophysiology is a method to study the function of electrically active properties of cells. It is widely used in both clinical applications, such as electroencephalograms (EEGs) and research applications, such as the monitoring of single ion channels. A large amount of the research on the physiology and electrical activity of neurons and their networks is performed at the cellular level, and knowledge of common microscopy techniques is essential.

  • Voltage Imaging

    All animal cells are surrounded by a cell membrane, composed of a double layer of lipids with proteins embedded in it, as seen in Fig.1. Standard electricity (such as in your home) mainly involves the movement of electrons; but electrophysiology involves the movement of ions (atoms that have a charge). In the body, some of the most common ions are sodium (Na+), potassium (K+), calcium (Ca2+) and chlorine (Cl–).

  • Single-Molecule FRET

    The lab of Prof. Keith Weninger develops single-molecule fluorescence methods to study biomolecular systems, with a particular focus on FRET to study proteins involved in DNA mismatch repair. Prof. Weninger further explained his research, “I do single-molecule FRET experiments on tethered DNA molecules with surface-immobilized TIRF microscopy.”

  • Light Sheet and Single Molecule Tracking

    The Cambridge Advanced Imaging Centre (CAIC) at the University of Cambridge develops modern imaging techniques to answer some of the most pressing and challenging biological questions. Keeping in mind the needs and demands of biologists, one of the current developments is a localization based 3D super-resolution microscope

  • Single Molecule TIRF

    The Klenerman group at the University of Cambridge investigates intracellular signalling in T-cells, a vital component of the human adaptive immune response. They are particularly interested in the kinetic-segregation model of T-cell signalling which proposes that signalling is only possible when CD45 molecules on the T-cell surface are sterically excluded from the T-cell receptor site.

  • What Is Neuroscience?

    Neuroscience is the study of the nervous system, and is one of the most interdisciplinary and rapidly‑advancing scientific fields, with branches in computer science, medicine, biology, and psychology. The areas of study within neuroscience are also broad, from more abstract concepts such as thought, emotion, and memory to the behavioral characteristics of all animals such as sleep, movement, socialization and also the breakdown of these systems during disease.

  • How Is An Image Made?

    Cameras are incredible tools that allow us to capture and make sense of the visible world around us. Most mobile phones made today come with a camera, meaning that more people than ever are becoming familiar with camera software and taking images.

  • Camera Test Protocol

    The detector is one of the most important components of any microscope system. Accurate detector readings are vital for collecting reliable biological data to process for publication.To ensure your camera is performing as well as it should be, Photometrics designed a range of tests that can be performed on any microscope.